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SelectComponents
ComponentMeasurements
DeleteBorderComponents
ImageMultiply

Image Processing
Life Sciences

Analyze Segmented Cells

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Use blob analysis techniques to mark the location and size of cells in a microscopy image

Use an image of cells under a microscope:

In[1]:=

i=

;

Pick out the individual cells that aren't overlapping:

In[2]:=

cells=SelectComponents[DeleteBorderComponents[Binarize[i,{0,.7}]],{"Area","Holes"},100<#1<1000&&#2>0&]

Out[2]=

Create red circles centered at the center of each cell, with roughly the same radius as the cells:

In[3]:=

circles=Graphics[{Red,Thick,Circle@@#&/@ComponentMeasurements[ImageMultiply[i,cells],{"Centroid","EquivalentDiskRadius"}]〚All,2〛}]

Out[3]=

Add the circles to the original microscopy image:

In[4]:=

Show[i,circles]

Out[4]=

Related Symbols

SelectComponents
ComponentMeasurements
DeleteBorderComponents
ImageMultiply

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